A Cohesin-Based Partitioning Mechanism Revealed upon Transcriptional Inactivation of Centromere.

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dc.contributor.author Tsabar, Michael
dc.contributor.author Haase, Julian
dc.contributor.author Harrison, Benjamin
dc.contributor.author Snider, Chloe E
dc.contributor.author Eldridge, Brittany
dc.contributor.author Kaminsky, Lila
dc.contributor.author Hine, Rebecca M
dc.contributor.author Haber, James E
dc.contributor.author Bloom, Kerry
dc.date.accessioned 2019-01-17T19:30:57Z
dc.date.available 2019-01-17T19:30:57Z
dc.date.issued 2016
dc.identifier.issn 1553-7404
dc.identifier.uri https://hdl.handle.net/10192/36125
dc.description.abstract Transcriptional inactivation of the budding yeast centromere has been a widely used tool in studies of chromosome segregation and aneuploidy. In haploid cells when an essential chromosome contains a single conditionally inactivated centromere (GAL-CEN), cell growth rate is slowed and segregation fidelity is reduced; but colony formation is nearly 100%. Pedigree analysis revealed that only 30% of the time both mother and daughter cell inherit the GAL-CEN chromosome. The reduced segregation capacity of the GAL-CEN chromosome is further compromised upon reduction of pericentric cohesin (mcm21Ã ), as reflected in a further diminishment of the Mif2 kinetochore protein at GAL-CEN. By redistributing cohesin from the nucleolus to the pericentromere (by deleting SIR2), there is increased presence of the kinetochore protein Mif2 at GAL-CEN and restoration of cell viability. These studies identify the ability of cohesin to promote chromosome segregation via kinetochore assembly, in a situation where the centromere has been severely compromised.
dc.format.extent 1 file
dc.language English
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.isversionof https://dx.doi.org/10.1371/journal.pgen.1006021
dc.rights Creative Commons Attribution 4.0 International License
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Research Article
dc.subject Anaphase
dc.subject Cell Processes
dc.subject Chemical Compounds
dc.subject QH426-470
dc.subject Physical Sciences
dc.subject Genetics
dc.subject Organic Chemistry
dc.subject Chromosome Biology
dc.subject Chemistry
dc.subject Carbohydrates
dc.subject Chromosome Structure and Function
dc.subject Glucose
dc.subject Biology and Life Sciences
dc.subject Chromosomes
dc.subject Galactose
dc.subject Chromosomal Disorders
dc.subject Cell Biology
dc.subject Clinical Genetics
dc.subject Centromeres
dc.subject Monosaccharides
dc.subject Organic Compounds
dc.subject Metaphase
dc.subject Medicine and Health Sciences
dc.subject Cell Cycle and Cell Division
dc.title A Cohesin-Based Partitioning Mechanism Revealed upon Transcriptional Inactivation of Centromere.
dc.type Article
dc.contributor.department Department of Biology
dc.relation.journal PLoS Genetics
dc.identifier.pmid 27128635
dc.identifier.pmcid PMC4851351
dc.description.esploro yes


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