Position effects influencing intrachromosomal repair of a double-strand break in budding yeast

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dc.contributor.author Harber, James
dc.contributor.author Lee, Cheng-Sheng
dc.contributor.author Wang, Ruoxi W.
dc.date.accessioned 2017-10-31T18:04:15Z
dc.date.available 2017-10-31T18:04:15Z
dc.date.issued 2017-07-11
dc.identifier.citation PLoS One12(7: e0180994 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180994
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10192/34677
dc.description Published article can be found at: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0180994
dc.description.abstract Repair of a double-strand break (DSB) by an ectopic homologous donor sequence is subject to the three-dimensional arrangement of chromosomes in the nucleus of haploid budding yeast. The data for interchromosomal recombination suggest that searching for homology is accomplished by a random collision process, strongly influenced by the contact probability of the donor and recipient sequences. Here we explore how recombination occurs on the same chromosome and whether there are additional constraints imposed on repair. Specifically, we examined how intrachromosomal repair is affected by the location of the donor sequence along the 813-kb chromosome 2 (Chr2), with a site-specific DSB created on the right arm (position 625 kb). Repair correlates well with contact frequencies determined by chromosome conformation capture-based studies (r = 0.85). Moreover, there is a profound constraint imposed by the anchoring of the centromere (CEN2, position 238 kb) to the spindle pole body. Sequences at the same distance on either side of CEN2 are equivalently constrained in recombining with a DSB located more distally on one arm, suggesting that sequences on the opposite arm from the DSB are not otherwise constrained in their interaction with the DSB. The centromere constraint can be partially relieved by inducing transcription through the centromere to inactivate CEN2 tethering. In diploid cells, repair of a DSB via its allelic donor is strongly influenced by the presence and the position of an ectopic intrachromosomal donor.
dc.description.sponsorship Funded by the Brandeis University Open Access Fund and the NIH grant GM20056 to JEH.
dc.language English
dc.language.iso eng
dc.publisher PLoS
dc.rights Copyright by authors 2017
dc.subject Centromeres
dc.subject DNA repair
dc.subject Homologous chromosomes
dc.subject Gene conversion
dc.subject Telomeres
dc.title Position effects influencing intrachromosomal repair of a double-strand break in budding yeast
dc.type Article
dc.contributor.department Department of Biology
dc.identifier.doi https://doi.org/10.1371/journal.pone.0180994
dc.description.esploro yes


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