The stringent response and cell cycle arrest in Escherichia coli.

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dc.contributor.author Ferullo, Daniel J
dc.contributor.author Lovett, Susan T
dc.date.accessioned 2019-01-29T18:18:27Z
dc.date.available 2019-01-29T18:18:27Z
dc.date.issued 2008
dc.identifier.issn 1553-7390
dc.identifier.issn 1553-7404
dc.identifier.uri https://hdl.handle.net/10192/36383
dc.description.abstract The bacterial stringent response, triggered by nutritional deprivation, causes an accumulation of the signaling nucleotides pppGpp and ppGpp. We characterize the replication arrest that occurs during the stringent response in Escherichia coli. Wild type cells undergo a RelA-dependent arrest after treatment with serine hydroxamate to contain an integer number of chromosomes and a replication origin-to-terminus ratio of 1. The growth rate prior to starvation determines the number of chromosomes upon arrest. Nucleoids of these cells are decondensed; in the absence of the ability to synthesize ppGpp, nucleoids become highly condensed, similar to that seen after treatment with the translational inhibitor chloramphenicol. After induction of the stringent response, while regions corresponding to the origins of replication segregate, the termini remain colocalized in wild-type cells. In contrast, cells arrested by rifampicin and cephalexin do not show colocalized termini, suggesting that the stringent response arrests chromosome segregation at a specific point. Release from starvation causes rapid nucleoid reorganization, chromosome segregation, and resumption of replication. Arrest of replication and inhibition of colony formation by ppGpp accumulation is relieved in seqA and dam mutants, although other aspects of the stringent response appear to be intact. We propose that DNA methylation and SeqA binding to non-origin loci is necessary to enforce a full stringent arrest, affecting both initiation of replication and chromosome segregation. This is the first indication that bacterial chromosome segregation, whose mechanism is not understood, is a step that may be regulated in response to environmental conditions.
dc.format.extent 1 file
dc.language English
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.isversionof https://dx.doi.org/10.1371/journal.pgen.1000300
dc.rights Creative Commons Attribution 4.0 International License
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Bacterial Outer Membrane Proteins
dc.subject Cell Cycle
dc.subject Chromosome Segregation
dc.subject Chromosomes, Bacterial
dc.subject DNA-Binding Proteins
dc.subject Escherichia coli
dc.subject Escherichia coli Proteins
dc.subject Mutation
dc.subject Origin Recognition Complex
dc.subject Replication Origin
dc.subject Site-Specific DNA-Methyltransferase (Adenine-Specific)
dc.subject Bacterial Outer Membrane Proteins
dc.title The stringent response and cell cycle arrest in Escherichia coli.
dc.type Article
dc.contributor.department Department of Biology
dc.relation.journal PLoS Genetics
dc.identifier.pmid 19079575
dc.identifier.pmcid PMC2586660
dc.description.esploro yes


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