Nascent-Seq reveals novel features of mouse circadian transcriptional regulation.

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dc.contributor.author Menet, Jerome S
dc.contributor.author Rodriguez, Joseph
dc.contributor.author Abruzzi, Katharine C
dc.contributor.author Rosbash, Michael
dc.date.accessioned 2019-01-17T19:30:53Z
dc.date.available 2019-01-17T19:30:53Z
dc.date.issued 2012
dc.identifier.issn 2050-084X
dc.identifier.uri https://hdl.handle.net/10192/36106
dc.description.abstract A substantial fraction of the metazoan transcriptome undergoes circadian oscillations in many cells and tissues. Based on the transcription feedback loops important for circadian timekeeping, it is commonly assumed that this mRNA cycling reflects widespread transcriptional regulation. To address this issue, we directly measured the circadian dynamics of mouse liver transcription using Nascent-Seq (genome-wide sequencing of nascent RNA). Although many genes are rhythmically transcribed, many rhythmic mRNAs manifest poor transcriptional rhythms, indicating a prominent contribution of post-transcriptional regulation to circadian mRNA expression. This analysis of rhythmic transcription also showed that the rhythmic DNA binding profile of the transcription factors CLOCK and BMAL1 does not determine the transcriptional phase of most target genes. This likely reflects gene-specific collaborations of CLK:BMAL1 with other transcription factors. These insights from Nascent-Seq indicate that it should have broad applicability to many other gene expression regulatory issues.DOI:http://dx.doi.org/10.7554/eLife.00011.001.
dc.format.extent 1 file
dc.language English
dc.language.iso eng
dc.publisher eLife Sciences Publications Ltd.
dc.relation.isversionof https://dx.doi.org/10.7554/eLife.00011
dc.rights Creative Commons Attribution 3.0 Unported
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject Circadian rhythms
dc.subject Mouse
dc.subject RNA processing
dc.subject high-throughput sequencing
dc.subject nascent RNA
dc.subject post-transcriptional regulation
dc.subject transcription
dc.subject ARNTL Transcription Factors
dc.subject Animals
dc.subject CLOCK Proteins
dc.subject Circadian Rhythm
dc.subject DNA
dc.subject Gene Expression Profiling
dc.subject Gene Expression Regulation
dc.subject Light
dc.subject Liver
dc.subject Male
dc.subject Mice
dc.subject Mice, Inbred C57BL
dc.subject Protein Binding
dc.subject RNA, Messenger
dc.title Nascent-Seq reveals novel features of mouse circadian transcriptional regulation.
dc.type Article
dc.contributor.department Department of Biology
dc.relation.journal eLife
dc.identifier.pmid 23150795
dc.identifier.pmcid PMC3492862
dc.description.esploro yes


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