Zds1/Zds2–PP2A(Cdc55) complex specifies signaling output from Rho1 GTPase

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dc.contributor.author Jonasson, Erin M.
dc.contributor.author Rossio, Valentina
dc.contributor.author Hatakeyama, Riko
dc.contributor.author Abe, Mitsuhiro
dc.contributor.author Ohya, Yoshikazu
dc.contributor.author Yoshida, Satoshi
dc.date.accessioned 2017-12-08T20:52:54Z
dc.date.available 2017-12-08T20:52:54Z
dc.date.issued 2016-01-04
dc.identifier.citation J. Cell Biol. Vol. 212 No. 1 51–61 en_US
dc.identifier.issn 0021-9525
dc.identifier.issn 1540-8140
dc.identifier.uri http://hdl.handle.net/10192/35058
dc.description Published version can be found on the Journal Cell Biology's site: http://doi.org/10.1083/jcb.201508119 en_US
dc.description.abstract Budding yeast Rho1 guanosine triphosphatase (GTPase) plays an essential role in polarized cell growth by regulating cell wall glucan synthesis and actin organization. Upon cell wall damage, Rho1 blocks polarized cell growth and repairs the wounds by activating the cell wall integrity (CWI) Pkc1–mitogen-activated protein kinase (MAPK) pathway. A fundamental question is how active Rho1 promotes distinct signaling outputs under different conditions. Here we identified the Zds1/Zds2–protein phosphatase 2ACdc55 (PP2ACdc55) complex as a novel Rho1 effector that regulates Rho1 signaling specificity. Zds1/Zds2–PP2A(Cdc55) promotes polarized growth and cell wall synthesis by inhibiting Rho1 GTPase-activating protein (GAP) Lrg1 but inhibits CWI pathway by stabilizing another Rho1 GAP, Sac7, suggesting that active Rho1 is biased toward cell growth over stress response. Conversely, upon cell wall damage, Pkc1–Mpk1 activity inhibits cortical PP2A(Cdc55), ensuring that Rho1 preferentially activates the CWI pathway for cell wall repair. We propose that PP2A(Cdc55)specifies Rho1 signaling output and that reciprocal antagonism between Rho1–PP2A(Cdc55) and Rho1–Pkc1 explains how only one signaling pathway is robustly activated at a time. en_US
dc.description.sponsorship Supported by the Sprout grant from Brandeis University, an American-Italian Cancer Foundation Postdoctoral fellowship and a Massachusetts Life Sciences Center grant. en_US
dc.language English
dc.language.iso eng
dc.publisher Rockefeller University Press en_US
dc.rights Copyright by the authors 2016 en_US
dc.subject Cell growth en_US
dc.subject Yeast en_US
dc.title Zds1/Zds2–PP2A(Cdc55) complex specifies signaling output from Rho1 GTPase en
dc.type Article en_US
dc.identifier.doi http://doi.org/10.1083/jcb.201508119 S en_US


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