Contralateral Inactivation of dorsal Hippocampus and medial Prefrontal Cortex using DREADDs Impairs Spatial Learning

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dc.contributor.advisor Jadhav, Shantanu
dc.contributor.author Dai, Yu
dc.date.accessioned 2017-05-22T20:23:54Z
dc.date.available 2017-05-22T20:23:54Z
dc.date.issued 2017
dc.identifier.uri http://hdl.handle.net/10192/33882
dc.description.abstract Hippocampus and prefrontal cortex individually are both known to be important in storing and retrieving memories to guide behaviors. Studies showing the functional overlap, anatomical connection and coordination of neuronal network activity patterns of these two regions indicate that hippocampus and prefrontal cortex communicate with each other to support learning, working memory formation and memory guided decision-making. In this research project, we aim to test the hypothesis that perturbing ipsilateral interactions between dorsal hippocampus (dHPC) and media prefrontal cortex (mPFC) will impair the rats’ abilities for learning and retrieving spatial memories. A chemogenetic approach, DREADDs (Designer Receptors Exclusively Activated by Designer Drugs), was used to inactivate the target brain regions in rats learning a W-track continuous spatial alternation task. This hippocampal-dependent task is comprised of two different components that depend on different types of memory. In order to test whether or not this task is also mPFC dependent, and whether or not ipsilateral interactions between dHPC and mPFC are important for this task, we implemented a multi-day spatial alternation task paradigm with separable phases of learning and retrieval after task acquisition. We found that the learning process of the outbound component of this task, but not the inbound component, was significantly impaired if the interactions between dHPC and mPFC were impaired, or mPFC was inactivated bilaterally.
dc.description.sponsorship Brandeis University, Graduate School of Arts and Sciences
dc.format.mimetype application/pdf
dc.language English
dc.language.iso eng
dc.publisher Brandeis University
dc.relation.ispartofseries Brandeis University Theses and Dissertations
dc.rights Copyright by Yu Dai 2017
dc.title Contralateral Inactivation of dorsal Hippocampus and medial Prefrontal Cortex using DREADDs Impairs Spatial Learning
dc.type Thesis
dc.contributor.department Interdepartmental Program in Neuroscience
dc.degree.name MS
dc.degree.level Masters
dc.degree.discipline Neuroscience
dc.degree.grantor Brandeis University, Graduate School of Arts and Sciences


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