Insight into the activation mechanisms of Src, Abl and their ancestors

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dc.contributor.advisor Kern, Dorothee
dc.contributor.author Biswas, Sarita
dc.date.accessioned 2016-05-12T18:42:11Z
dc.date.available 2016-05-12T18:42:11Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/10192/32349
dc.description Joint BS/MS Program
dc.description.abstract Src and Abl are two tyrosine kinases that have similar structures but different activation and inhibition mechanisms. Previous studies and crystal structures have given insight on the structures of Abl and Src which include information on vital tyrosine residues, regulatory domains and myristoyl groups. In order to investigate these regulatory mechanisms and their evolution over time, we reconstructed a common ancestor of Src and Abl and the common ancestors between Abl/Src and the Tec and Fer families of kinases. We wanted to create a methodology to examine the evolution of kinase regulation not only qualitatively, but also quantitatively. We started with the well-known Src and Abl, and compared their abilities to autophosphorylate and their rates of activity via a kinetic assay that measured kcat, the number of conversions of substrate molecule per second by a single active site. We manipulated different regulatory elements, so that we could investigate the differential modes of regulation of these kinases. Once these methods were well developed, we repeated them on early ancestors of Src and Abl, so that we could study how regulation had evolved over time.
dc.description.sponsorship Brandeis University, Graduate School of Arts and Sciences
dc.format.mimetype application/pdf
dc.language English
dc.language.iso eng
dc.publisher Brandeis University
dc.relation.ispartofseries Brandeis University Theses and Dissertations
dc.rights Copyright by Sarita Biswas 2016
dc.title Insight into the activation mechanisms of Src, Abl and their ancestors
dc.type Thesis
dc.contributor.department Department of Biology
dc.degree.name MS
dc.degree.level Masters
dc.degree.discipline Biochemistry
dc.degree.grantor Brandeis University, Graduate School of Arts and Sciences


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