Effect of Chromosome Tethering on Nuclear Organization in Yeast

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dc.contributor.author Avşaroğlu, Bariş
dc.contributor.author Bronk, Gabriel
dc.contributor.author Gordon-Messer, Susannah
dc.contributor.author Ham, Jungoh
dc.contributor.author Bressan, Debra A.
dc.contributor.author Haber, James E.
dc.contributor.author Kondev, Jane
dc.date.accessioned 2016-02-04T23:24:02Z
dc.date.available 2016-02-04T23:24:02Z
dc.date.issued 2014-07-14
dc.identifier.citation Avşaroğlu B, Bronk G, Gordon-Messer S, Ham J, Bressan DA, Haber JE, et al. (2014) Effect of Chromosome Tethering on Nuclear Organization in Yeast. PLoS ONE 9(7): e102474. doi:10.1371/journal.pone.0102474
dc.identifier.uri http://hdl.handle.net/10192/31552
dc.description.abstract Interphase chromosomes in Saccharomyces cerevisiae are tethered to the nuclear envelope at their telomeres and to the spindle pole body (SPB) at their centromeres. Using a polymer model of yeast chromosomes that includes these interactions, we show theoretically that telomere attachment to the nuclear envelope is a major determinant of gene positioning within the nucleus only for genes within 10 kb of the telomeres. We test this prediction by measuring the distance between the SPB and the silent mating locus (HML) on chromosome III in wild–type and mutant yeast strains that contain altered chromosome-tethering interactions. In wild-type yeast cells we find that disruption of the telomere tether does not dramatically change the position of HML with respect to the SPB, in agreement with theoretical predictions. Alternatively, using a mutant strain with a synthetic tether that localizes an HML-proximal site to the nuclear envelope, we find a significant change in the SPB-HML distance, again as predicted by theory. Our study quantifies the importance of tethering at telomeres on the organization of interphase chromosomes in yeast, which has been shown to play a significant role in determining chromosome function such as gene expression and recombination.
dc.description.sponsorship Funded by the Brandeis University Open Access Fund, NSF grants MRSEC 0820492 and DMR-1206146, and by the NIH grant GM20056.
dc.language English
dc.language.iso eng
dc.publisher PLoS
dc.rights Copyright by the authors 2014
dc.subject Telomeres
dc.subject Chromosomes
dc.subject Polymers
dc.subject Genetic loci
dc.subject Yeast and fungal models
dc.subject Chromosome structure and function
dc.subject Probability distribution
dc.subject Saccharomyces cerevisiae
dc.title Effect of Chromosome Tethering on Nuclear Organization in Yeast
dc.type Article
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0102474

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