Individual IKs Channels at the Surface of Mammalian Cells Contain Two KNCE1 Accessory Subunits

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dc.contributor.author Plant, Leigh D.
dc.contributor.author Xiong, Dazhi
dc.contributor.author Dai, Hui
dc.contributor.author Goldstein, Steve A. N.
dc.date.accessioned 2016-02-02T21:50:17Z
dc.date.available 2016-02-02T21:50:17Z
dc.date.issued 2014-03-03
dc.identifier.citation Leigh D. Plant, Dazhi Xiong, Hui Dai, and Steve A. N. Goldstein Individual IKs channels at the surface of mammalian cells contain two KCNE1 accessory subunits PNAS 2014 111 (14) E1438-E1446; published ahead of print March 3, 2014, doi:10.1073/pnas.1323548111
dc.identifier.issn 1091-6490
dc.identifier.other 1091-6490
dc.identifier.uri http://hdl.handle.net/10192/31512
dc.description This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1323548111/-/DCSupplemental.
dc.description.abstract KCNE1 (E1) β-subunits assemble with KCNQ1 (Q1) voltage-gated K+ channel α-subunits to form IKslow (IKs) channels in the heart and ear. The number of E1 subunits in IKs channels has been an issue of ongoing debate. Here, we use single-molecule spectroscopy to demonstrate that surface IKs channels with human subunits contain two E1 and four Q1 subunits. This stoichiometry does not vary. Thus, IKs channels in cells with elevated levels of E1 carry no more than two E1 subunits. Cells with low levels of E1 produce IKs channels with two E1 subunits and Q1 channels with no E1 subunits—channels with one E1 do not appear to form or are restricted from surface expression. The plethora of models of cardiac function, transgenic animals, and drug screens based on variable E1 stoichiometry do not reflect physiology.
dc.description.sponsorship This work was supported by the Brandeis University Open Access Fund
dc.language English
dc.language.iso eng
dc.publisher Proceedings of the National Academy of Sciences of the United States of America
dc.rights Copyright by the authors 2014
dc.subject minK
dc.subject LQTS
dc.subject Single-particle fluorimetry
dc.subject Kv7.1
dc.subject KvLQT1
dc.title Individual IKs Channels at the Surface of Mammalian Cells Contain Two KNCE1 Accessory Subunits
dc.type Article
dc.contributor.department Department of Biochemistry
dc.identifier.doi www.pnas.org/cgi/doi/10.1073/pnas.1323548111
dc.description.esploro yes


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