Failure to reproduce reversal of hippocampal LTP by the PKCinhibitor, ZIP

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dc.contributor.advisor Lisman, John Schur, Solon 2014-10-03T16:36:45Z 2014-10-03T16:36:45Z 2010
dc.description.abstract At the cellular level, some patterns of excitation of neurons have been found to induce long-term increases in synaptic strength between neurons, tlu·ough a process termed long-tem1 potentiation (L TP). While the mechanism of LTP induction is well understood, the mechanisms that lead to the maintenance of synaptic strengthening are not as well understood. PKM( is an isoform of the PKC superfamily and is claimed to be necessary for late-phase LTP maintenance. The main evidence for the role of PKM1; was inferred from reports that L TP can be reduced by the myristoylated 1; -pseudosubstrate peptide (ZTP), claimed to inhibit PKq spccii1cally. Our objectives in this study were to produce stable L TP recordings for up to three hours after induction in an open chamber. This allowed us to test whether we could reproduce ZIP-induced L TP reversal. Measurements were made in the rat CA I region of the hippocampus, as measured by Held recordings. Our experiments have shown that we have successfully induced late phase long-term potentiation in the stratum radiatum of the. CA I region in the hippocampus. However, we were not been able to produce L TP reversal by adding ZIP one hour after LTP induction. We can therefore conclude that furth!!r research should focus on ZIP specificity and the reproducibility of ZIP induced L TP reversal.
dc.format.mimetype application/pdf
dc.language English
dc.language.iso eng
dc.publisher Brandeis University
dc.relation.ispartofseries Brandeis University Theses and Dissertations
dc.rights Copyright Solon Schur 2010
dc.title Failure to reproduce reversal of hippocampal LTP by the PKCinhibitor, ZIP
dc.type Thesis
dc.contributor.department Undergraduate Program in Biology BS Bachelors Biology Brandeis University, College of Arts and Sciences

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