Regulation of Spindle Checkpoint Protein Bfa1 by Mitotic Phosphorylation

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dc.contributor.advisor Yoshida, Satoshi
dc.contributor.author Paulissen, Eric
dc.date.accessioned 2014-05-19T15:06:52Z
dc.date.available 2014-05-19T15:06:52Z
dc.date.issued 2014
dc.identifier.uri http://hdl.handle.net/10192/27072
dc.description.abstract In budding yeast, the timing of mitotic exit is tightly controlled by a canonical signaling cascade called the Mitotic Exit Network (MEN). MEN is activated by the GTPase Tem1, which is inhibited by a Bfa1-Bub2 GAP complex. It is known that the Polo-like kinase Cdc5 phosphorylates and inactivates Bfa1 to promote mitotic exit and cytokinesis, however, the mechanism that allows Bfa1 phosphorylation by Cdc5 specifically during late mitosis is not well understood. I made a specific hypothesis that mitotic Cdk is preventing Bfa1 phosphorylation by Cdc5 in metaphase to prevent precocious mitotic exit. We have identified six potential Cdk-dependent phosphorylation sites in Bfa1 and made an unphosphorylatable bfa1 mutant (bfa1-6A). In my experiments, the bfa1-6A mutant was not defective in mitotic arrest or exhibited dominant effects in mitotic exit in the conditions tested. I will discuss physiological relevance of possible roles of Cdk in Bfa1 regulations and future experiments.
dc.description.sponsorship Brandeis University, Graduate School of Arts and Sciences
dc.format.mimetype application/pdf
dc.language English
dc.language.iso eng
dc.publisher Brandeis University
dc.relation.ispartofseries Brandeis University Theses and Dissertations
dc.rights Copyright by Eric Paulissen 2014
dc.subject Mitosis
dc.subject Molecular Biology
dc.subject Mitotic Exit
dc.subject Cell Cycle
dc.title Regulation of Spindle Checkpoint Protein Bfa1 by Mitotic Phosphorylation
dc.type Thesis
dc.contributor.department Graduate Program in Molecular and Cell Biology
dc.degree.name MS
dc.degree.level Masters
dc.degree.discipline Molecular and Cell Biology
dc.degree.grantor Brandeis University, Graduate School of Arts and Sciences


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