Regulation of Spindle Checkpoint Protein Bfa1 by Mitotic Phosphorylation

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dc.contributor.advisor Yoshida, Satoshi en_US
dc.contributor.author Paulissen, Eric
dc.date.accessioned 2014-05-19T15:06:52Z
dc.date.available 2014-05-19T15:06:52Z
dc.date.issued 2014
dc.identifier.uri http://hdl.handle.net/10192/27072
dc.description.abstract In budding yeast, the timing of mitotic exit is tightly controlled by a canonical signaling cascade called the Mitotic Exit Network (MEN). MEN is activated by the GTPase Tem1, which is inhibited by a Bfa1-Bub2 GAP complex. It is known that the Polo-like kinase Cdc5 phosphorylates and inactivates Bfa1 to promote mitotic exit and cytokinesis, however, the mechanism that allows Bfa1 phosphorylation by Cdc5 specifically during late mitosis is not well understood. I made a specific hypothesis that mitotic Cdk is preventing Bfa1 phosphorylation by Cdc5 in metaphase to prevent precocious mitotic exit. We have identified six potential Cdk-dependent phosphorylation sites in Bfa1 and made an unphosphorylatable bfa1 mutant (bfa1-6A). In my experiments, the bfa1-6A mutant was not defective in mitotic arrest or exhibited dominant effects in mitotic exit in the conditions tested. I will discuss physiological relevance of possible roles of Cdk in Bfa1 regulations and future experiments. en_US
dc.description.sponsorship Brandeis University, Graduate School of Arts and Sciences en_US
dc.format.mimetype application/pdf en_US
dc.language English en_US
dc.language.iso eng en_US
dc.publisher Brandeis University en_US
dc.relation.ispartofseries Brandeis University Theses and Dissertations
dc.rights Copyright by Eric Paulissen 2014 en_US
dc.subject Mitosis en_US
dc.subject Molecular Biology en_US
dc.subject Mitotic Exit en_US
dc.subject Cell Cycle en_US
dc.title Regulation of Spindle Checkpoint Protein Bfa1 by Mitotic Phosphorylation en_US
dc.type Thesis en_US
dc.contributor.department Graduate Program in Molecular and Cell Biology en_US
dc.degree.name MS en_US
dc.degree.level Masters en_US
dc.degree.discipline Molecular and Cell Biology en_US
dc.degree.grantor Brandeis University, Graduate School of Arts and Sciences en_US


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