The molecular basis for regulation of Drosophila Nervous wreck

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dc.contributor.advisor Rodal, Avital Messelaar, Emily 2013-05-23T20:15:36Z 2013-05-23T20:15:36Z 2013
dc.description.abstract Synaptic growth at the Drosophila melanogaster neuromuscular junction (NMJ) depends on the endocytosis and downstream intracellular trafficking of signaling receptors. Nervous wreck (Nwk) is an F-BAR/SH3 protein that regulates trafficking of the signaling receptors in motor neuron terminals to attenuate neuronal growth. Little is known about how Nwk’s trafficking activity is regulated in vivo to achieve precise signal-specific activity. Here I show that Nwk is autoinhibited by an intramolecular interaction between its F-BAR domain and its SH3b domain. I show that the SH3b ligand Dynamin-associating protein 160 kD (Dap160) does not relieve this autoinhibition in cell culture. I also show that the SH3b domain of Nwk, though required, is not sufficient for interaction with Dap160 and that Dap160 has an affinity for the F-BAR domain of Nwk. I propose that the tertiary folding of Nwk reveals the binding sequence for Dap160. In the future, I will further investigate the mechanisms of intra- and intermolecular regulation of Nwk in order to better understand how Nwk controls healthy neuronal development.
dc.format.mimetype application/pdf
dc.language English
dc.language.iso eng
dc.publisher Brandeis University
dc.relation.ispartofseries Brandeis University Theses and Dissertations
dc.rights Copyright by Emily Messelaar 2013
dc.title The molecular basis for regulation of Drosophila Nervous wreck
dc.type Thesis
dc.contributor.department Undergraduate Program in Biology BS Bachelors Biology Brandeis University, College of Arts and Sciences

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